Biological reagents - Bovine viral diarrhoea

Biological reagents image

Bovine viral diarrhoea is a viral disease of cattle and other ruminants caused by the Bovine Viral Diarrhoea Virus (BVDV), which is a member of the pestivirus genus.

There are four recognised species within the pestivirus genus: BVDV-1, BVDV-2, Border Disease Virus (BDV) of sheep and Classical Swine Fever Virus (CSFV).

Monoclonal antibodies for BVDV and BDV

Proficiency Testing Services are available for reagents below

Antibodies - Monoclonal (mAb) - Murine origin

Pan-pestivirus reactive monoclonal antibodies

 

mAb

antigen

immunoglobulin class

detects

 

RAE0801

WB103

NS2-3

IgG1

virtually all pestivirus

1mg

 

WB105

NS2-3

IgG2a

virtually all pestivirus

1mg

A pool of two non-competing monoclonal antibodies, which have reacted with all pestiviruses tested at APHA to date, including BVD, CSF and BD viruses, as well as uncharacterised viruses from other ruminants. They are suitable for indirect labelling techniques for the detection of pestivirus antigens in cell cultures and tissue sections. Store at 4°C.

BVDV monoclonal antibodies

 

mAb

antigen

immunoglobulin class

detects

 

RAE2020

WB103

NS2-3

IgG1

all pestivirus

1mg

 

WB112

NS2-3

IgG2a

all pestivirus

1mg

 

WB166

E2

IgG1

BVDV type 1 & BDV

1mg

 

WB214

E2

IgG1

BVD type 1

1mg

A pool of four monoclonal antibodies, which detects a wider range of BVDV antigens in tissue cultures. This pool is preferable to RAE0801 for detecting bovine pestiviruses. Store at 4°C.

BVDV and BDV monoclonal antibodies

 

mAb

antigen

immunoglobulin class

detects

 

RAE0822

WB162

E2

IgG1

BVDV-1

1mg

RAE0823

WB210

Erns

IgG1

BVDV-1

1mg

RAE0824

WB215

E2

IgG2b

BVDV-1

1mg

RAE4174

WB214

E2

IgG1

BVDV-1

1mg

RAE0241

WB160

NS2-3

IgG1

BVDV-1 & BDV

1mg

RAE4173

WB166

E2

IgG1

BVDV-1 & BDV

1mg

RAE0993

WS538

E2

IgG2a

BVDV-2

1mg

RAE0992

WS433

Erns

IgG2a

BVDV-2 & BDV

1mg

RAE0825

WS363

Erns

IgG1

BDV

1mg

RAE4751

WS373

Erns

IgG1

BDV

1mg

RAE4750

WS371

Erns

IgG1

BDV

1mg

Ruminant pestiviruses can be divided into BVDV1, BVDV2 and BDV. No single monoclonal antibody binds specifically to all three subtypes but the above panel taken together will recognise most strains and indicate to which subtype they belong. None of these monoclonal antibodies bind to the CSF virus, so the panel may be used in conjunction with RAE0242 or RAE0826 for the differentiation of BVD/BD and CSF isolates from pigs, as recommended by the European Community. Store at 4°C.

Further discriminatory mAbs are available on request.

References:
Edwards S, Sands J J, Paton D J (1991) Vet. Microbiol 29, 101-108.
Paton D J, Sands J J, Edwards S (1994) Arch.Virol. 135, 241-252.
Edwards S, Sands J J, Harkness J W (1988) Arch.Virol. 102,197-206.

Antisera

RAB0042 BVDV positive serum 2ml

A positive control for serum antibody assays, antigen detection procedures and within research applications. Freeze-dried, hyper-immune bovine serum derived from new born calves. Store at 4°C or at -20°C after reconstitution.

Monoclonal antibodies for CSFV

 

mAb

antigen

immunoglobulin class

detects

 

RAE0242

WH211

E2

IgG1

CSF

1mg

RAE0826

WH303

E2

IgG1

CSF

1mg

Reference:
Lin M, Mallory M, Clavijo A (2000) J Virol 74, 11619 - 11625

NB: CSFV monoclonal antibodies are not available in the UK without prior approval of Defra – please contact the Customer Service Team for further details.

To discuss your requirements please contact our Customer Services Team

Tel:  +44 (0)3000 600001
Email: salesdesk@apha.gov.uk

Page last modified: 28 November, 2018